Featured Scientist

Regina working as a student assistant at the Pesticide Investigations Unit. © Regina Donohoe, all rights reserved.

Regina collects grunion eggs at El Capitan State Beach after a major Santa Barbara oil spill. © Regina Donohoe, all rights reserved.

Crew sampling in Tyvek suits following the Santa Barbara oil spill. © Regina Donohoe, all rights reserved.

Regina Donohoe is an OSPR staff toxicologist working in the Resource Restoration Program. As an ecotoxicologist, she combines the methods of ecology and toxicology to evaluate the effects of pollutants on fish, wildlife and their habitats.

For the past 17 years, Regina has been involved in the remediation of hazardous waste sites, petroleum production facilities and military bases to ensure that chemical contamination is cleaned up to levels that are protective of our natural resources. Regina also provides ecotoxicology support for spill response and assesses the injuries resulting from spills as part of the natural resource damage assessment team.

Regina received a Ph.D. in toxicology from Oregon State University, a M.S. in ecology from San Diego State University and a B.S. in environmental toxicology from the University of California at Davis.

Who or what inspired you to become a scientist?

One summer day when I was a bored five-year-old, my mom asked me to roam our foothills property and bring back every different kind of leaf I could find. It was a clever mom move to keep me busy all afternoon but it is my earliest memory of being fascinated with nature. However, it wasn’t until I met my high school biology teacher that I knew I wanted to be a scientist. Her classroom was a museum filled with skeletons, shells, plants and aquaria of fish, and that class set me on the path to major in science in college. Later, my graduate school advisors shared their passion for science and kept me going.

What got you interested in working with fish and wildlife?

While trying to decide on an undergraduate major, I took an introduction to environmental toxicology class. The professor told the grisly story of the dancing cats of Minimata Bay, Japan during the 1950s. After mercury-laden wastewater was released into the Bay, the fish accumulated it and when the cats ate the fish, they began to walk erratically (or “dance”) because the mercury poisoned their nervous system. It was an “aha” moment. The fact that a single chemical could disrupt an entire ecosystem intrigued and saddened me at the same time, inspiring me to declare environmental toxicology as my major and the focus of my career.

What brought you to CDFW? What inspires you to stay?

It was 1982 when I took a year-long Student Assistant job at the Pesticide Investigation Unit Laboratory of the then-California Department of Fish and Game. The job duties included “creamer patrol,” picking up dead carp from agricultural drains in the Sacramento Valley for pesticide analysis. On a 100° F day, the fish decayed so quickly that when they were scooped up in the net they often exploded into a creamy mess. Another aspect of the job was to conduct bioassays with fish to determine what levels of pesticides in the water were safe. I left the position to study the effects of sewage discharges on the algal populations of Tijuana estuary, meeting my husband while knee-deep in mud. After that, I researched how chemicals could act like hormones, with environmental estrogens altering reproduction in fish. This led to an ecotoxicologist job at the California Environmental Protection Agency. A friend recommended I apply for a job at OSPR and 17 years later, I returned to where I had started.

What is a typical day like for you at work?

My work moves in a couple of different gears. On the slower gear, a ten-year timeframe, I work on remediation of military bases and hazardous waste sites. For each site, the nature and extent of chemical contamination is characterized, the method and level of cleanup is decided, cleanup occurs and then there is follow-up monitoring. For example, we have removed lead bullets from sand dune firing ranges, metal plating waste from salt marshes and crude oil from grasslands. Throughout the multi-year process for each site, I make many visits, read reports, attend meetings and provide comments related to ecotoxicology.

When spills happen, everything shifts into high gear. I pull together information about the spill, plan and conduct studies and assist wherever help is needed. After the spill response ends, the natural resource damage assessment process continues but at a less frenetic pace. I analyze data, prepare presentations and write up results.

In between, I am involved in training and planning, teaching classes on the effects of oil on ecosystems and reviewing literature to be better prepared for the next spill.

Over the course of your career, was there a discovery or an incident that surprised you?

When we were called to respond to the oil spill in Santa Barbara in 2015, we had to plan for fish sampling one day and carry it out the following day. Usually, it might take a couple of weeks to organize such a sampling event. But people from multiple agencies volunteered their gear and help, others quickly purchased needed supplies, experts flew in to collect tissue samples and folks drove many miles to be there on short notice. The sampling day began early, including gearing up in bulky protective clothing to fish on the beach, and ended with a late night of data intake. Everyone brought a positive attitude and worked hard to get the samples we needed to support the natural resource damage assessment. We achieved a near-impossible task because everyone gave it their all. The power of people working together truly surprised and amazed me.

What do you enjoy most about working in ecotoxicology?

California has incredibly diverse ecosystems – deserts, grasslands, chaparral, forests, rivers, wetlands, estuaries and marine habitat. Spills happen all over California, providing me opportunities to investigate impacts to many plant and animal species in a wide variety of habitats and spill scenarios. For example, I’ve been asked: How does diesel affect an old growth redwood tree? What levels of metals are of concern to California Tiger Salamanders? Is brine harmful to ephemeral streams? How does crude oil impact kelp forests? These questions make me scratch my head and think, and learn new things every day. That, to me, is the best thing about being a scientist.

If you had free reign and unlimited funding, what scientific project would you most like to do?

When scientists began to evaluate how oil spills affected ecosystems in the 1970s, they did some interesting laboratory and field studies, but they were limited by the analytical chemistry and assessment methods available at the time. In many cases, oil concentrations in water or tissues were not measured or were simply quantified as total hydrocarbons. Today, we know that oil is a mixture of hundreds to thousands of chemicals that we can quantify at very low levels in the environment. We also have new biochemical and genetic tools that can detect subtle changes in animals in response to oil exposure. It would be great to repeat many of those earlier studies using our current technologies to further our understanding of how oil affects ecosystems, improving our ability to respond to spills and restore habitats following spills.

Do you have any advice for people considering careers in science or natural resources?

Follow that innate scent trail. What makes work seem like play? What keeps you going when you lose the trail? What rewards you at the end of the day? If you have a passion for science or the natural resources, make it your career and be sure to stop and smell all those interesting scents along the way.

Photo © Regina Donohoe, all rights reserved
Top photo: Regina, conducting rocky intertidal monitoring on San Francisco Bay

Krysta Rogers is a senior environmental scientist at CDFW’s Wildlife Investigations Laboratory. She is the statewide lead for avian mortality investigations. Her professional experience has been focused almost exclusively on birds, including bird identification, biology, ecology and behavior. She has led and participated in numerous field projects including bird surveys, trapping, banding and sample collection.

Krysta earned both her Bachelors and Masters of Science degrees in Wildlife from Humboldt State University. She came to CDFW in 2007 as a wildlife biologist, starting out as the avian influenza surveillance coordinator. She subsequently worked on developing the lab’s Avian Investigations program, which includes mortality investigations, disease research and health surveillance for the many different bird species in California.

Who or what inspired you to become a scientist?

I had an interest in animals from a very young age and pursued this initially in the field of veterinary medicine. Although I enjoyed the animal health aspect, I learned I did not want to become a veterinarian in a small animal practice. I was unaware of other career possibilities until I took an elective course on endangered species at my local community college. The class introduced me to the career of wildlife biologist which subsequently informed my decision to attend Humboldt State University to obtain a degree in Wildlife. Later, while working toward my Master’s degree, my thesis advisor, Dr. Rick Botzler, taught me how to incorporate my interest in animal health into the study of wildlife biology.

What is a typical day like for you at work?

As the lead for avian mortality investigations, I spend most of my time responding to reports of dead birds from CDFW staff, other agencies, wildlife rehabilitation centers and the public. Given that most birds are active during the day and are thus highly visible to the public, these reports come in almost constantly. For investigations, I obtain carcasses for post-mortem exam to determine the cause of death, which may inform management actions. I either will perform the gross necropsy myself and submit various tissues for testing, or I will coordinate with another lab for the post-mortem exam and testing. Then I interpret the results and determine the next course of action. These investigations are varied – for instance, house finches and mourning doves dying from diseases at backyard bird feeders, juvenile California scrub jays and Cooper’s hawks dying of West Nile virus, Western and Clark’s grebes dying from starvation along the coast and at inland reservoirs, or bald and golden eagles dying of toxicosis or electrocution.

What is your favorite species to interact with or study?

It’s impossible to pick a single species; I’m interested in birds in general. There are more than 600 species of birds in California that live in a diversity of habitats. Different diseases or conditions can affect different species. This great diversity means always having the opportunity to learn something new in terms of species biology, a pathogen or environmental impacts.

What is it about the work you do that you find most interesting?

Investigating causes of mortality is almost like being a detective. You need to gather lots of detail and determine what factors are relevant to the cause of death for a particular animal. Sometimes this may be relatively easy with a pathogen or condition you routinely investigate, but sometimes it’s something entirely new. This aspect keeps the investigations interesting.

What is the most challenging part of your job?

One challenge is communicating the importance of incorporating animal health into resource management and conservation plans. For many avian species, we don’t have even a basic understanding of the impacts of diseases or contaminants that may influence population growth or decline. The lack of this information may limit our ability to manage species over the long-term, especially in the face of climate change. Climate change will almost certainly favor certain pathogens or host species that can aid in disease transmission.

What is the most rewarding project that you’ve worked on for CDFW?

So far, it’s been collaborating with researchers at the University of California, Davis to investigate avian trichomonosis in our native band-tailed pigeons. Avian trichomonosis is a disease caused by a protozoan parasite that in band-tailed pigeons causes near annual large-scale mortality events. This project enabled us to genetically characterize the parasites infecting band-tailed pigeons, including naming a new species of parasite, and evaluate the ecological drivers and the population impacts of these disease events on band-tailed pigeons, a migratory game species.

If you had free reign and unlimited funding, what scientific project would you most like to do?

I’d like to work towards an improved understanding of the impacts certain pathogens have on different avian species. This would include genetically characterizing the pathogens, identifying host range, and learning how avian species interact with their environment and the means by which they are exposed to certain pathogens.

Do you have any advice for people considering careers in science or natural resources?

Because there are so many career options in the field of science and natural resources, I’d recommend trying to get exposed to a diversity of people and projects as early as possible (e.g. junior high, high school). This can be accomplished through interviewing professionals, volunteering, internships and paid jobs. Learning about the different career options enables you to determine your interests and will help guide your educational path and eventually your career goals.