Featured Scientist

Regina working as a student assistant at the Pesticide Investigations Unit. © Regina Donohoe, all rights reserved.

Regina collects grunion eggs at El Capitan State Beach after a major Santa Barbara oil spill. © Regina Donohoe, all rights reserved.

Crew sampling in Tyvek suits following the Santa Barbara oil spill. © Regina Donohoe, all rights reserved.

Regina Donohoe is an OSPR staff toxicologist working in the Resource Restoration Program. As an ecotoxicologist, she combines the methods of ecology and toxicology to evaluate the effects of pollutants on fish, wildlife and their habitats.

For the past 17 years, Regina has been involved in the remediation of hazardous waste sites, petroleum production facilities and military bases to ensure that chemical contamination is cleaned up to levels that are protective of our natural resources. Regina also provides ecotoxicology support for spill response and assesses the injuries resulting from spills as part of the natural resource damage assessment team.

Regina received a Ph.D. in toxicology from Oregon State University, a M.S. in ecology from San Diego State University and a B.S. in environmental toxicology from the University of California at Davis.

Who or what inspired you to become a scientist?

One summer day when I was a bored five-year-old, my mom asked me to roam our foothills property and bring back every different kind of leaf I could find. It was a clever mom move to keep me busy all afternoon but it is my earliest memory of being fascinated with nature. However, it wasn’t until I met my high school biology teacher that I knew I wanted to be a scientist. Her classroom was a museum filled with skeletons, shells, plants and aquaria of fish, and that class set me on the path to major in science in college. Later, my graduate school advisors shared their passion for science and kept me going.

What got you interested in working with fish and wildlife?

While trying to decide on an undergraduate major, I took an introduction to environmental toxicology class. The professor told the grisly story of the dancing cats of Minimata Bay, Japan during the 1950s. After mercury-laden wastewater was released into the Bay, the fish accumulated it and when the cats ate the fish, they began to walk erratically (or “dance”) because the mercury poisoned their nervous system. It was an “aha” moment. The fact that a single chemical could disrupt an entire ecosystem intrigued and saddened me at the same time, inspiring me to declare environmental toxicology as my major and the focus of my career.

What brought you to CDFW? What inspires you to stay?

It was 1982 when I took a year-long Student Assistant job at the Pesticide Investigation Unit Laboratory of the then-California Department of Fish and Game. The job duties included “creamer patrol,” picking up dead carp from agricultural drains in the Sacramento Valley for pesticide analysis. On a 100° F day, the fish decayed so quickly that when they were scooped up in the net they often exploded into a creamy mess. Another aspect of the job was to conduct bioassays with fish to determine what levels of pesticides in the water were safe. I left the position to study the effects of sewage discharges on the algal populations of Tijuana estuary, meeting my husband while knee-deep in mud. After that, I researched how chemicals could act like hormones, with environmental estrogens altering reproduction in fish. This led to an ecotoxicologist job at the California Environmental Protection Agency. A friend recommended I apply for a job at OSPR and 17 years later, I returned to where I had started.

What is a typical day like for you at work?

My work moves in a couple of different gears. On the slower gear, a ten-year timeframe, I work on remediation of military bases and hazardous waste sites. For each site, the nature and extent of chemical contamination is characterized, the method and level of cleanup is decided, cleanup occurs and then there is follow-up monitoring. For example, we have removed lead bullets from sand dune firing ranges, metal plating waste from salt marshes and crude oil from grasslands. Throughout the multi-year process for each site, I make many visits, read reports, attend meetings and provide comments related to ecotoxicology.

When spills happen, everything shifts into high gear. I pull together information about the spill, plan and conduct studies and assist wherever help is needed. After the spill response ends, the natural resource damage assessment process continues but at a less frenetic pace. I analyze data, prepare presentations and write up results.

In between, I am involved in training and planning, teaching classes on the effects of oil on ecosystems and reviewing literature to be better prepared for the next spill.

Over the course of your career, was there a discovery or an incident that surprised you?

When we were called to respond to the oil spill in Santa Barbara in 2015, we had to plan for fish sampling one day and carry it out the following day. Usually, it might take a couple of weeks to organize such a sampling event. But people from multiple agencies volunteered their gear and help, others quickly purchased needed supplies, experts flew in to collect tissue samples and folks drove many miles to be there on short notice. The sampling day began early, including gearing up in bulky protective clothing to fish on the beach, and ended with a late night of data intake. Everyone brought a positive attitude and worked hard to get the samples we needed to support the natural resource damage assessment. We achieved a near-impossible task because everyone gave it their all. The power of people working together truly surprised and amazed me.

What do you enjoy most about working in ecotoxicology?

California has incredibly diverse ecosystems – deserts, grasslands, chaparral, forests, rivers, wetlands, estuaries and marine habitat. Spills happen all over California, providing me opportunities to investigate impacts to many plant and animal species in a wide variety of habitats and spill scenarios. For example, I’ve been asked: How does diesel affect an old growth redwood tree? What levels of metals are of concern to California Tiger Salamanders? Is brine harmful to ephemeral streams? How does crude oil impact kelp forests? These questions make me scratch my head and think, and learn new things every day. That, to me, is the best thing about being a scientist.

If you had free reign and unlimited funding, what scientific project would you most like to do?

When scientists began to evaluate how oil spills affected ecosystems in the 1970s, they did some interesting laboratory and field studies, but they were limited by the analytical chemistry and assessment methods available at the time. In many cases, oil concentrations in water or tissues were not measured or were simply quantified as total hydrocarbons. Today, we know that oil is a mixture of hundreds to thousands of chemicals that we can quantify at very low levels in the environment. We also have new biochemical and genetic tools that can detect subtle changes in animals in response to oil exposure. It would be great to repeat many of those earlier studies using our current technologies to further our understanding of how oil affects ecosystems, improving our ability to respond to spills and restore habitats following spills.

Do you have any advice for people considering careers in science or natural resources?

Follow that innate scent trail. What makes work seem like play? What keeps you going when you lose the trail? What rewards you at the end of the day? If you have a passion for science or the natural resources, make it your career and be sure to stop and smell all those interesting scents along the way.

Photo © Regina Donohoe, all rights reserved
Top photo: Regina, conducting rocky intertidal monitoring on San Francisco Bay

Wildlife training with a mountain lion at CDFW’s Wildlife Investigations Lab outside of Sacramento.


John climbs redwoods at Hendy Woods State Park in Mendocino County.


Wildlife biologist by day, rock guitarist by night in the band Sticky’s Backyard.


John with wife, Trish, and daughters Phoenix (left) and Sequoia.


Mountain biker John near Lake Almanor.


Snowboarders’ expert runs have the best views

Wildlife biologist John Krause is a 17-year CDFW employee who serves Marin, Alameda and Contra Costa counties. In the heavily populated San Francisco Bay Area, human-wildlife issues often dominate his workday. But his professional pride and joy is the Eden Landing Ecological Reserve near Hayward. John has spent his career overseeing the restoration of 6,400 acres of commercial salt ponds to tidal marsh and other natural habitat while creating access and recreational opportunities for the public.

A native of Carmichael in Sacramento County, John holds a degree in terrestrial plant ecology from UC Davis.

Do you find it ironic that you are a wildlife biologist working in some of the most urbanized communities in the country?

Sure, at times. When I took this job back in 2001, I did not really know how much of the human dimension aspect of things I would be getting into. It’s a regular part of the job, dealing with the public about everything from “Where can I go hunt?” to “I’ve got a problem with a coyote in my backyard.”

How much of your time is spent dealing with human-wildlife conflicts?

It’s every day. A lot of it is depredation-related calls from the public – wild pigs, wild turkeys in the urban-wildlife interface, occasionally deer out in the vineyards causing property damage. There are regular calls about coyotes being perceived as a public safety risk, though coyotes are really more of a risk to domestic animals like cats, small dogs and backyard, free-ranging chickens. The number of these incidents is definitely increasing, primarily because we have open space immediately adjacent to these metropolitan areas. Many of these communities are tucked into the natural landscape so they are inextricably linked to the landscape.

Many people today just don’t have the background or understanding about the behavior of these wild critters. Our general message is to leave these critters be. But when wildlife becomes a nuisance, then it’s time to step up and make an effort to discourage that behavior. That might mean building a coop for your free-range chickens, hazing a coyote out of a neighborhood or thinking carefully about the kind of landscaping you are installing in your yard.

What prompted your interest in science and the outdoors?

I grew up on the American River. I had friends who lived right out there on the bluffs so as kids we were out there all the time biking around and hiking around, swimming in the river, going fishing.

I was a pre-med student originally. I thought I was going to go to professional school to be a dentist. My motivation as a kid was “I’ll be a dentist and I’ll be rich!” But I realized over time that wasn’t for me. I didn’t want to spend my life looking into people’s mouths. So I got into this career by thinking about what it was that I really cared about.

What brought you to CDFW and what inspires you to stay?

I was a biologist for Caltrans for two and a half years before I came to the department. I learned a lot at Caltrans – really useful stuff like reading plans and working with engineers that has served me well over the years with the wetland restoration work I do now. I was out on construction jobs in the Santa Cruz Mountains and there were all these issues coming up with listed species. It was great training. But ultimately, I wanted to work for a conservation agency instead of doing conservation work for a transportation agency.

This job was advertised and I was all over it. Counting deer and elk by helicopter or by driving out to remote areas to survey? Working in and managing wetlands for waterfowl and shorebirds or endangered mice? Counting rails by airboat? Yes, please! The work is really diverse, and I think that’s what keeps me so engaged. And I have this really cool project I get to work on – my legacy project, Eden Landing. I will hand it off to somebody else at some point and they will have a whole career finishing it off.

What is special about the Eden Landing Ecological Reserve?

It’s part of the largest wetlands restoration on the West Coast – 15,100 acres in the South Bay. It’s what I studied in college. Landscape change over time. We are restoring salt ponds to tidal marshes, keeping some managed ponds that birds have come to rely upon. It’s a 50-year project that started in 2003. I’m the guy on the ground working with all of our contractors and partners.

The water birds are the real stars of the show out there. They are the poster species for nature. We manage the ponds for the different seasons and bird species. I will go out and take a dry pond that has been set aside for snowy plover nesting in the spring, flood it up in late summer and watch the bugs come back. And a couple of weeks later the shorebirds show up and are taking advantage of it. And then later in the year we transition from shorebirds to ducks and we start flooding it up a little more for ducks. We’ve got shallower ponds for the dabblers and deeper water for the diving ducks.

Is there public access for birders and others at Eden Landing?

Absolutely. Public access is part of our mandate. We’ve got 4 miles of trails. We’ve got anglers out there. We’ve got kayaking and a kayak launch out there. I started the waterfowl hunting program there and we are just wrapping up our 14th waterfowl season. It’s a success in many ways.

What’s the story behind the waterfowl hunting program? It’s unique in that it is free, for one thing, and you allow hunting on some non-typical shoot days such as Tuesdays and Thursdays.

Hunters have to buy their license and state and federal duck stamps, but they don’t have to buy a lands pass for Eden Landing. San Francisco Bay has a long history and tradition of duck hunting and we wanted to continue that at Eden Landing. When Cargill owned the property, they leased out ponds and hunters built duck blinds and had duck hunting out there for decades. When CDFW took it over, we made it accessible to the public. We now host about 10 hunt days annually.

The South Bay federal wildlife refuges allow hunting on Wednesdays, Saturdays and Sundays. I wanted to provide more opportunities for hunters when those wildlife areas are closed so I added shoot days on Tuesdays and Thursdays. We get a lot of local hunters, and we see new hunters every year. Eden is getting pretty well-known in the hunting community, and our averages are pretty good for people coming out and getting birds – better than the wildlife areas and refuges a lot of the time. The hunters really appreciate that their dollars are supporting the restoration and enhancement of Bay-Delta wetlands.

Away from work, where are we likely to find you?

My wife and I have two young girls, 13 and 11. I’m an outdoor sports person. I mountain bike all the time. I go hiking. I love snowboarding. I’ve been snowboarding since 1984 – back before snowboarding was even a thing. I love to travel. My wife and I have been all over the world and have visited countries in Europe, Central America and Africa. More recently, my family has traveled in America, Mexico and Canada.

Tell us something about yourself many people would be surprised to learn.

I’m in a band. I play rhythm guitar and sing back-up vocals with friends I met in college. The band is called Sticky’s Backyard – Sticky was the nickname of a guy in Davis and we played our first gig in his backyard. That was 26 years ago, and we are still together. We play all original music. Jam rock is the best way I can describe it. We played the Lucasfilm employee holiday party in December. It’s a fun outlet – scientist by day, rocker by night. Sometimes those days and nights blend together.

John Krause photos
Top photo: John welcomes U.S. Senator Dianne Feinstein to the South Bay’s salt ponds in 2010, where native habitat was being restored and public access opened for the first time.

Morgan Knechtle is an environmental scientist with CDFW’s Northern Region in Yreka. He works on the Klamath River Project, which has a primary focus of estimating the return of adult salmon and steelhead to the Klamath and Trinity rivers. He is responsible for multiple field projects that manage salmonids in the Klamath River Basin, such as operating adult salmonid counting stations and coordinating adult spawning ground surveys on the Shasta River, Scott River and Bogus Creek, three highly productive salmonid tributaries to the Klamath River in Siskiyou County. Knechtle also assists with adult recovery efforts, which involve collecting biological information from returning adult salmon at Iron Gate Hatchery, and serves as one of CDFW’s technical representatives for the Klamath Dam Decommissioning Project, which involves the proposed elimination of four hydroelectric dams in northern California and Southern Oregon.

Knechtle earned a Bachelor of Science degree in freshwater fisheries from Humboldt State University and got his first job with CDFW as a scientific aide in the Russian River watershed. He was hired permanently in 2000 and spent four years working on salmonid life cycle monitoring stations on the Mendocino coast. Since 2004, he has worked with salmonids in the Klamath Basin, both on the Trinity River and in the tributaries to the Klamath River in Siskiyou County.

Who or what inspired you to become a scientist?

My love for rivers inspired me to become a scientist. During college at Humboldt State University, I was spending all of my free time fishing for salmon and steelhead and came to the realization that I could study these animals and make a living working with them.

The ability to be an advocate for fishery resources brought me to CDFW. CDFW is one of the only places a scientist can work with fisheries and truly be an objective voice for the resource. Many other organizations do not have this luxury.

What is a typical day like for you at work?

It depends on the time of year. During the fall and winter when adult salmonids are returning to the Klamath River, my world is extremely busy running and participating in multiple field projects monitoring the return of these amazing species. During the spring and summer, I spend much more time in the office crunching numbers and writing reports.

My Chinook salmon work focuses on providing information that can help accurately forecast abundance. This enables us to provide fishing opportunities while maintaining enough fish in the river for future generations. My coho salmon monitoring work focuses on providing accurate abundance information to track the status and trends of this endangered species over time.

As a technical expert on the Klamath Dam Decommissioning Project, in cooperation with other technical experts from other state and federal agencies, I help minimize effects to aquatic species inhabiting the Klamath River during the decommissioning phase of the project. Additionally, I participate in post-dam removal planning projects, including creating plans on how to implement the Iron Gate Hatchery post dam removal and coordinate with Oregon scientists on the reintroduction of salmon above Iron Gate Dam, with a goal of ensuring the recovery of salmonids and aquatic species above the project area.

What is the most rewarding project that you have worked on for CDFW?

Although the project is not yet complete, the Klamath Dam Decommissioning Project has the potential to be the most rewarding project I have worked on. It stands to be the largest river restoration project to ever be completed in North America, and given that status, as one can imagine, the project has a lot of moving parts. The potential benefits to salmonids in the Klamath and the improvements to the health of the river itself could be enormous. The long-term predicted improvements to water quality, habitat availability, natural flow dynamics and restoration of natural processes to the Klamath River will improve conditions, for not only anadromous salmon and steelhead but also the rest of the plant and animal community that depend on the river for part or all of their life history.

What is the most challenging aspect of your career as an environmental scientist?

It is extremely challenging when social and political concerns get mixed in with natural resource management. Working with coho salmon in the Klamath Basin has been very challenging due to its listed status and the fact that their abundance is extremely low.

If you had free reign and unlimited funding, what scientific project would you most like to do?

With unlimited funding, I would like to track and monitor the recovery of spring Chinook in the upper Klamath River post dam removal. They are nearly extinct and they are thought to once have been the most abundant species in the Klamath River Basin.

What aspect of working on the Klamath River is particularly challenging or rewarding?

Multiple stakeholders -- which include Native American tribes, federal and state trustee agencies, and freshwater and ocean anglers -- in the Klamath Basin make some aspects of salmon management challenging. However, when progress is made to restore the river, it is also extremely rewarding because you know that groups with very different perspectives have come together, negotiated agreement and reached consensus on difficult issues.

What is your favorite species to interact with or study?

Steelhead trout are my favorite species to work with. Steelhead are the most elusive of the Klamath salmonids and their complex life history make them a very difficult species to study. They are also my favorite fish to catch.

Any advice for people considering careers in science or natural resources?

The most obvious is for folks to stay in school and graduate from college. In addition, volunteering and interning in their field of interest early in their education is a benefit to get a taste of what the career might really be like.

CDFW photos of Morgan Knechtle working along the Shasta River.