Featured Scientist

Regina working as a student assistant at the Pesticide Investigations Unit. © Regina Donohoe, all rights reserved.

Regina collects grunion eggs at El Capitan State Beach after a major Santa Barbara oil spill. © Regina Donohoe, all rights reserved.

Crew sampling in Tyvek suits following the Santa Barbara oil spill. © Regina Donohoe, all rights reserved.

Regina Donohoe is an OSPR staff toxicologist working in the Resource Restoration Program. As an ecotoxicologist, she combines the methods of ecology and toxicology to evaluate the effects of pollutants on fish, wildlife and their habitats.

For the past 17 years, Regina has been involved in the remediation of hazardous waste sites, petroleum production facilities and military bases to ensure that chemical contamination is cleaned up to levels that are protective of our natural resources. Regina also provides ecotoxicology support for spill response and assesses the injuries resulting from spills as part of the natural resource damage assessment team.

Regina received a Ph.D. in toxicology from Oregon State University, a M.S. in ecology from San Diego State University and a B.S. in environmental toxicology from the University of California at Davis.

Who or what inspired you to become a scientist?

One summer day when I was a bored five-year-old, my mom asked me to roam our foothills property and bring back every different kind of leaf I could find. It was a clever mom move to keep me busy all afternoon but it is my earliest memory of being fascinated with nature. However, it wasn’t until I met my high school biology teacher that I knew I wanted to be a scientist. Her classroom was a museum filled with skeletons, shells, plants and aquaria of fish, and that class set me on the path to major in science in college. Later, my graduate school advisors shared their passion for science and kept me going.

What got you interested in working with fish and wildlife?

While trying to decide on an undergraduate major, I took an introduction to environmental toxicology class. The professor told the grisly story of the dancing cats of Minimata Bay, Japan during the 1950s. After mercury-laden wastewater was released into the Bay, the fish accumulated it and when the cats ate the fish, they began to walk erratically (or “dance”) because the mercury poisoned their nervous system. It was an “aha” moment. The fact that a single chemical could disrupt an entire ecosystem intrigued and saddened me at the same time, inspiring me to declare environmental toxicology as my major and the focus of my career.

What brought you to CDFW? What inspires you to stay?

It was 1982 when I took a year-long Student Assistant job at the Pesticide Investigation Unit Laboratory of the then-California Department of Fish and Game. The job duties included “creamer patrol,” picking up dead carp from agricultural drains in the Sacramento Valley for pesticide analysis. On a 100° F day, the fish decayed so quickly that when they were scooped up in the net they often exploded into a creamy mess. Another aspect of the job was to conduct bioassays with fish to determine what levels of pesticides in the water were safe. I left the position to study the effects of sewage discharges on the algal populations of Tijuana estuary, meeting my husband while knee-deep in mud. After that, I researched how chemicals could act like hormones, with environmental estrogens altering reproduction in fish. This led to an ecotoxicologist job at the California Environmental Protection Agency. A friend recommended I apply for a job at OSPR and 17 years later, I returned to where I had started.

What is a typical day like for you at work?

My work moves in a couple of different gears. On the slower gear, a ten-year timeframe, I work on remediation of military bases and hazardous waste sites. For each site, the nature and extent of chemical contamination is characterized, the method and level of cleanup is decided, cleanup occurs and then there is follow-up monitoring. For example, we have removed lead bullets from sand dune firing ranges, metal plating waste from salt marshes and crude oil from grasslands. Throughout the multi-year process for each site, I make many visits, read reports, attend meetings and provide comments related to ecotoxicology.

When spills happen, everything shifts into high gear. I pull together information about the spill, plan and conduct studies and assist wherever help is needed. After the spill response ends, the natural resource damage assessment process continues but at a less frenetic pace. I analyze data, prepare presentations and write up results.

In between, I am involved in training and planning, teaching classes on the effects of oil on ecosystems and reviewing literature to be better prepared for the next spill.

Over the course of your career, was there a discovery or an incident that surprised you?

When we were called to respond to the oil spill in Santa Barbara in 2015, we had to plan for fish sampling one day and carry it out the following day. Usually, it might take a couple of weeks to organize such a sampling event. But people from multiple agencies volunteered their gear and help, others quickly purchased needed supplies, experts flew in to collect tissue samples and folks drove many miles to be there on short notice. The sampling day began early, including gearing up in bulky protective clothing to fish on the beach, and ended with a late night of data intake. Everyone brought a positive attitude and worked hard to get the samples we needed to support the natural resource damage assessment. We achieved a near-impossible task because everyone gave it their all. The power of people working together truly surprised and amazed me.

What do you enjoy most about working in ecotoxicology?

California has incredibly diverse ecosystems – deserts, grasslands, chaparral, forests, rivers, wetlands, estuaries and marine habitat. Spills happen all over California, providing me opportunities to investigate impacts to many plant and animal species in a wide variety of habitats and spill scenarios. For example, I’ve been asked: How does diesel affect an old growth redwood tree? What levels of metals are of concern to California Tiger Salamanders? Is brine harmful to ephemeral streams? How does crude oil impact kelp forests? These questions make me scratch my head and think, and learn new things every day. That, to me, is the best thing about being a scientist.

If you had free reign and unlimited funding, what scientific project would you most like to do?

When scientists began to evaluate how oil spills affected ecosystems in the 1970s, they did some interesting laboratory and field studies, but they were limited by the analytical chemistry and assessment methods available at the time. In many cases, oil concentrations in water or tissues were not measured or were simply quantified as total hydrocarbons. Today, we know that oil is a mixture of hundreds to thousands of chemicals that we can quantify at very low levels in the environment. We also have new biochemical and genetic tools that can detect subtle changes in animals in response to oil exposure. It would be great to repeat many of those earlier studies using our current technologies to further our understanding of how oil affects ecosystems, improving our ability to respond to spills and restore habitats following spills.

Do you have any advice for people considering careers in science or natural resources?

Follow that innate scent trail. What makes work seem like play? What keeps you going when you lose the trail? What rewards you at the end of the day? If you have a passion for science or the natural resources, make it your career and be sure to stop and smell all those interesting scents along the way.

Photo © Regina Donohoe, all rights reserved
Top photo: Regina, conducting rocky intertidal monitoring on San Francisco Bay

OSPR’s Jim Hughes and Annie Nelson evaluate an oil spill exercise at the Larkspur Ferry Terminal.


Annie Nelson and Ima Doty on the Schnyder dock in Eureka


Annie Nelson atop Donner Pass, where OSPR has responded to two past pipeline oil spills

Annie Nelson is in her second year as CDFW’s Office of Spill Prevention and Response (OSPR) specialist for best achievable technology, focusing on mechanical oil spill response equipment. She began her career with OSPR as a scientific aid a few months after earning her Bachelor of Science degree in wildlife management from Humboldt State University. She gained permanent employee status as an analyst, working in the Natural Resource Damage Assessment Program. She found it rewarding to witness and coordinate post-spill restoration efforts, but after about seven years, a move to the drills and exercises unit opened the door for her to the world of response preparedness as an environmental scientist. Establishing and fortifying that program took Nelson through the next eight years of her career, before accepting her current position.

What keeps her at OSPR are the wonderful people, the constant learning curve and the many unique opportunities the spill response world provides.

Who or what inspired you to become a scientist?

In 5th or 6th grade, the cutest boy in my class expressed that science was his favorite subject. That was the spark for me. Once I started exploring science, I realized it was really cool and fun to learn. I enjoyed biology and chemistry during high school and became hooked on understanding how our planet operates – down to the molecular level.

What got you interested in working with wildlife?

My senior year of high school came with the opportunity to participate in the Regional Occupational Program (ROP) and I chose “Animal Careers.” The ROP offered classroom education as well as hands-on experience in your field of choice. When it was time to choose jobsites, most of my fellow classmates in the animal careers course worked with veterinarians. I was fortunate enough to get a spot with Wild Things, Inc., an exotic animal facility near my home. I prepared diets and fed the animals, handled them for educational shows and cleaned up after them. I got to work with a variety of wildlife species, from big cats to primates to birds and reptiles – even a sea lion. When it was time to look at colleges and career opportunities, I combined my love for animals with science and studied wildlife at Humboldt State.

Who or what brought you to CDFW? What inspires you to stay?

My dad encouraged me to pursue a career with the state. CDFW was the first agency that came to mind. I applied for the warden cadet academy, wanting to enforce the laws that protect wildlife in California. Unfortunately, my vision disqualified me. I got my foot in the door with a CDFW scientific aid position in the Office of Spill Prevention and Response. I knew very little about oil spills at the time, but 17 years later I am a senior environmental scientist (specialist). My love for this team and continual learning since my first day on the job has kept me with OSPR.

What is a typical day like for you at work? Please describe a “day in the life.”

I usually spend my early morning hours researching online for any new articles that have come out regarding oil spill technology. My focus is mechanical equipment for containment and recovery, for example: boom, sorbents and skimmers. The goal is to evaluate as many as possible and develop a program for best practices. I also develop workshops. Right now, I’m helping to create an agenda for a workshop that focuses on response to spills of oils called bitumen. Bitumen is the consistency of peanut butter at room temperature and sinks in water, making cleanup difficult. This is an area that hasn’t had much advancement with technology but is a growing concern for responders.

As OSPR’s fisheries closure coordinator, I’m the contact person for calls during the work day from field staff who are either participating in an exercises or responding to actual spills for consultation regarding fishery closures due to oil spills. I gather information to provide the Office of Environmental Health Hazard Assessment (OEHHA), the agency that evaluates our information and provides recommendations on whether to close or keep the fishery open. Our agencies work together to determine whether a fisheries closure is necessary, where it will be, and how long it will last during oil spills. Currently, I am working on a presentation to take to our field responders that educates them about this process and their role in it. There is so much to learn, I am never bored!

What is the most rewarding project that you’ve worked on for CDFW?

In my second position at OSPR, I joined a brand new team that became the drills and exercises unit. Our role was to ensure that the oil industry in California was prepared to respond to spills. I helped build the program from the ground up, re-writing regulations and evaluating hundreds of response drills and exercises all over the state. Industry soon realized that we meant business and they were quick to comply. Improving spill preparedness through the relationships fostered between OSPR and the oil industry we regulate proved rewarding.

Over the course of your career, was there a discovery or an incident that surprised you?

The most jaw-dropping incident that has occurred during my career was the Deepwater Horizon oil spill in the Gulf of Mexico in 2010. The Macondo well head poured more than 3 billion gallons of crude oil into the Gulf from 5,000 feet below the surface. Eleven people lost their lives when the rig blew up, and it took 87 days to cap the well. This still blows my mind to think about. Although I wasn’t one of them, quite of few of my OSPR co-workers responded to the incident. It was a proud moment for the OSPR team to bring experience and resources to the disaster and help.

If you had free reign and unlimited funding, what scientific project would you most like to do?

I’ll stick with my current field. I’d love to conduct studies of the wildlife species most commonly injured by oil spills, in order to develop humane but highly effective hazing techniques and technology. In order to avoid mass wildlife casualties, quick containment of a spill is crucial; however, this doesn’t often occur. Hazing is sometimes necessary to keep animals away from the slick, but the effectiveness is hit-or-miss. I’d be interested in developing some new biology-based tools for hazing to reduce the need for rehabilitation (washing), which can be stressful on wildlife and take up resources.

What is the best thing about being a wildlife scientist?

Knowing that my work ultimately protects California’s wildlife from injury during oil spills is pretty great in itself, but my favorite part of the job is working directly with animals. It’s not every day that I get to handle a common murre or a grebe, but I’ve had opportunities to work in the oiled wildlife care facility in Cordelia, which brought me back to my high school days – feeding birds and cleaning cages. These experiences are always a treat.

Any advice for people considering careers in science or natural resources?

I think that career seekers need to ask themselves three questions: What is my passion, what am I good at, and what opportunities are out there? If you can match up at least two of these with your chosen career path, you have a good chance for success. What I love about the fields of science and natural resources is that our planet is always changing. We will never know everything there is to know and there will always be new discoveries and a need for scientists to study, protect and improve our environment.

Top photo: Annie Nelson (right) and Andrea Moore prepare to board a tug and observe an on-water boom deployment in El Segundo.

CDFW photos

Anna Burkholder is a senior environmental scientist with the Preparedness Branch of CDFW’s Office of Spill Prevention and Response (OSPR) in Sacramento. She is the statewide Inland Geographic Response Plan Coordinator, working with fellow OSPR staff throughout the state to develop inland response plans for waterways at high risk for an oil spill. She has worked for CDFW for 20 years, most recently joining OSPR in 2016. In addition to her role as response plan coordinator, she is training for two oil spill emergency response positions: wildlife branch director (the position that oversees wildlife response efforts during a spill) and liaison officer (which works to address stakeholders’ concerns during a spill).

Anna earned her Bachelor of Science degree in biology, with an emphasis in zoology, from San Francisco State University. She prefers being outdoors, hiking with her dogs, snowshoeing, paddle boarding and horseback riding. She volunteers with the DOVES Guidance Program, a therapeutic horseback riding program for at-risk kids, as well as for NorCal German Shorthaired Pointer Rescue. She is improving her skills at upland bird hunting, including pheasant and turkey, and is still waiting to take a shot at her first tom.

Who or what inspired you to become a scientist?

When the movie Jaws came out, I was both terrified and fascinated. To this day, it is my favorite movie and I am more thrilled than ever with sharks. I briefly met Peter Benchley, the author of Jaws, several years back at the Monterey Bay Aquarium and that was exciting. He was promoting a new book trying to dispel the terrifying image of Great Whites, which he felt partially responsible for creating. I was also inspired by Dr. John McCosker, a Great White Shark expert that works for the California Academy of Sciences in San Francisco. McCosker had begun investigating why shark attacks occur, assessing their danger to humans in the grand scheme of things. He has worked to help understand the importance of sharks in the ecosystem and how they relate to the health of our oceans.

What got you interested in working with wildlife?

Clearly I would have loved to study sharks but didn’t follow that path. Along the way though, some of my classes at San Francisco State got me interested in some aspects of wildlife. Studying the behavior of snow leopards at the San Francisco Zoo (they sleep a lot!) for my Animal Behavior class, and doing some mark and recapture studies of mice and voles in Pacifica for an Ecology class, were fun experiences.

Who or what brought you to CDFW? What inspires you to stay?

Pure luck brought me to CDFW. After I graduated from college, I was working for a biotech company in Hercules and wasn’t terribly happy with the work. I used to go for walks during my break time and look out over San Pablo Bay and think to myself, “I need a job out there.” While taking an oceanography class at night at a junior college, I was looking at the job board one evening and saw a posting for a temporary scientific aid position with CDFW, working on the Bay Study Project. I got the job and lo and behold, there I was out on a boat every month, sampling fish throughout the Delta and San Francisco Bay (including San Pablo Bay!). I have never looked back.

Twenty years later, I guess it was a good move for me. I stay because I love this department, mostly the people I work with, and the dedication and passion we all have for the environment and the strong desire to protect the species and habitats in the state.

What is a typical day like for you at work?

When I am not traveling to participate in oil spill drills, oil spill workshops or Incident Command System training, I spend time working on the Geographic Response Plan template document that will be used to produce regional plans throughout the state for oil spill response. I coordinate with my OSPR colleagues, as well as other state and federal agencies, oil spill response organizations and industry folks on the development of these documents so they can provide a useful tool in responding to an incident. It’s been great to meet and work with an entirely new set of folks that I haven’t come across in my career until now, and to have a common goal of preparing for oil spills and working to protect the public, the environment and economic resources in our state.

What is the most memorable or rewarding project that you’ve worked on for CDFW?

I’ve spent a lot of time in the field working on boats. I worked on the 2007 Pike Eradication project at Lake Davis, and even got to spend a day escorting Delta and Dawn, the wayward humpback whale cow and calf that swam up the deep water channel to the Port of Sacramento in 2007. We escorted them on the last day they were observed inside the Golden Gate as they made their way through San Pablo Bay and finally back out to the Pacific Ocean.

I would have to say the most rewarding project is shaping up to be my new job with OSPR. The office was established 25 years ago and has a very comprehensive marine program in terms of preparedness and response to oil spills, but since OSPR’s jurisdiction expanded to include inland in 2015, I get to be on the forefront of establishing preparedness plans to protect all waters of the state.

What do you enjoy most about your job?

I work with an amazing group of folks in every part of our department, and we have a common goal of preserving and enhancing the natural environment. Being able to feel proud of the department you work for and cheering on the achievements of others in your field is a great feeling. Not to mention some of the great days in the field, which include flying along the California coast to record data on nesting seabirds, looking for nesting grebes in high mountain lakes and touring the state’s bird and marine mammal rescue and rehabilitation facilities.

Is there a preconception about scientists you would like to dispel?

The term “environmental scientist” encompasses a wide range of job duties within the State of California, including field biologists and environmental planning and permitting staff. We certainly have state scientists who conduct important laboratory research, including folks who work for OSPR and conduct water analysis and DNA fingerprinting on oil products. And what’s wrong with a periodic table? I loved general chemistry class!

Do you have advice for people considering careers in science or natural resources?

If it is what you love to do, then don’t shy away from following that path. You can try different aspects of working in the natural resources field and then focus on what you enjoy the most. I would volunteer or take shorter-term assignments to work with multiple organizations and get experience in different areas. Meet experts in their field and get a foot in the door through internships or part-time jobs. There are so many exciting directions you can go in this field.