Follow these Steps for Proper Extraction CWD Sampling Kit contains a specimen bag, data card, and two sample bags. If a sampling kit is not available two resealable plastic freezer bags and a piece of paper or 3x5 card may be used. Please contact cwd@wildlife.ca.gov to request sample bags. Fill out data card. Record hunter’s first and last name, GOID, document # from the deer or elk tag (D- 0000000000-0), date, and location of the harvest (hunt zone at a minimum, GPS coordinates preferred). Record the name of the person extracting the lymph node. Place the data card in the outer pocket of the specimen bag or attached to the ear if submitting the head. If you do not have a sampling kit, a piece of paper or 3x5 card can be used to record the above information. We will not test samples that cannot be traced back to a specific location or a hunter. Collect both Retropharyngeal Lymph Nodes (RPLN) – For a trained individual this process can take less than a minute. If you wish to preserve the hide, cape out or skin the animal first. Lymph nodes are located at the base of the skull between the windpipe and the spine. With the deer laid on it's back, head and neck extended, make a cut just behind the jaw through the trachea and esophagus. The paired RPLNs should be located at the 5 and 7 o’clock position relative to the windpipe and are firm, oval to kidney shaped, tan to red tissues between the size of a dime and a quarter. Place each harvested RPLNs in separate sample bags provided. Place the two sample bags in the resealable compartment of the larger specimen bag. Samples can be frozen until submission to CDFW. If without a sample kit, place both in a single resealable plastic freezer bag. Seal that bag and place it within a second resealable plastic freezer bag along with the paper or 3x5 card containing the requested information. Videos of how to sample for RPLNs can be found online, below are links to the Wyoming Game and Fish and the Colorado Parks and Wildlife videos WGFD video(opens in new tab) CPW video(opens in new tab) Head collection for later sampling If you are unable to collect the lymph nodes, CDFW asks that you remove the head along with the topmost portion of the neck and attach a data card to the head with tape or zip tie. The head can then be frozen until it can be collected by CDFW. Sample Submission Please reach out to your local CDFW contact to arrange pick up of samples. RPLN samples can also be shipped directly to CDFW. If you reach out to one of the contacts below, they can arrange for a freezer shipping box and label to be sent to your location. For additional information please contact us at cwd@wildlife.ca.gov or (916) 358-2930. Ship Samples to this address: CWD Surveillance Program Wildlife Health Lab CA Department of Fish and Wildlife 1701 Nimbus Rd. Suite D Rancho Cordova, CA 95670 (916) 358-2790 Additional Resources CWD in North America(opens in new tab) CWD-Related Hunting Regulations(opens in new tab) Carcass Transport Regulations(opens in new tab) Report a Sick Deer Distribution of CWD in North America. Courtesy of USGS National Wildlife Health Center. CDFW Resources Chronic Wasting Disease Brochure (PDF)(opens in new tab) Folleto Sobre la Enfermedad del Desgaste Crónico (PDF)(opens in new tab) Recommendations to Meat Processors about CWD (PDF)(opens in new tab) Advanced Hunter Education Webinar on CWD (Video)(opens in new tab) External Resources CWD Alliance(opens in new tab) Colorado Parks and Wildlife – CWD Info and Testing(opens in new tab) Wyoming Game and Fish Department – Chronic Wasting Disease (CWD) in Wyoming Wildlife (opens in new tab) Cervid Carcass Regulations - North Carolina Wildlife Resources Commission(opens in new tab)